Method Development and Validation of Rifampicin by Using RP-HPLC Method in Pharmaceutical Formulations

A rapid and sensitive RP-HPLC method with UV detection (219nm) for routine analysis of Rifampicin in a pharmaceutical formulation was developed. Chromatography performed mobile phase containing methanol assay (99.8%) flow rate 0.9ml/min. Quantitation accomplished an internal standard method. The procedure validated linearity (correlation coefficient = 0.9957), accuracy limit (LOD) intraday precision. To test validation the three factors were considered as linearity, precision, LOD where phase, flowrate pressure are respectively selected methanol, 0.9ml/min, 1200 pascals. ensure precise measurements, variables taken into account analyst equipment. RSD value (0.20%) indicated good precision analytical proposed simple; highly sensitive, precise, accurate retention time less than 3 min indicating that is useful quality control.